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Table 4 TLR agonists up-regulate IL-6 release at 6 and 24 h in purified rat cortical microglia and enriched, but not microglia-depleted, astrocytes

From: Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes

Treatment Microglia (pg IL-6/well) Astrocytes (pg IL-6/well)
   - - - L-LME - - - L-LME
6 h 24 h 6 h 6 h 24 h 24 h
Control 0 0 0 0 3.5 ± 3.5 0
LPS 26.3 ± 3.1 34.4 ± 0.2 75.5 ± 3.1 0 123.0 ± 1.6 4.3 ± 2.2
Zymosan 29.3 ± 0.8 64.5 ± 4.3 90.4 ± 3.5 0 130.3 ± 4.3 5.5 ± 2.9
Poly(I:C) 12.0 ± 1.5 23.4 ± 2.3 49.7 ± 2.0 0 79.1 ± 3.6 7.7 ± 4.6
  1. Purified microglia and enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 and 24 h for IL-6 release into the culture medium by ELISA. Data are means ± SEM (n = 3). Note the lack of astrocyte responsiveness after L-LME.