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Table 4 TLR agonists up-regulate IL-6 release at 6 and 24 h in purified rat cortical microglia and enriched, but not microglia-depleted, astrocytes

From: Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes

Treatment

Microglia (pg IL-6/well)

Astrocytes (pg IL-6/well)

  

- - -

L-LME

- - -

L-LME

6 h

24 h

6 h

6 h

24 h

24 h

Control

0

0

0

0

3.5 ± 3.5

0

LPS

26.3 ± 3.1

34.4 ± 0.2

75.5 ± 3.1

0

123.0 ± 1.6

4.3 ± 2.2

Zymosan

29.3 ± 0.8

64.5 ± 4.3

90.4 ± 3.5

0

130.3 ± 4.3

5.5 ± 2.9

Poly(I:C)

12.0 ± 1.5

23.4 ± 2.3

49.7 ± 2.0

0

79.1 ± 3.6

7.7 ± 4.6

  1. Purified microglia and enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 and 24 h for IL-6 release into the culture medium by ELISA. Data are means ± SEM (n = 3). Note the lack of astrocyte responsiveness after L-LME.
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Journal of Neuroinflammation

ISSN: 1742-2094