Fig. 3

TCF-1 regulates IFNG and GZMB expression. a Binding region of TCF-1 in the promoter of IFNG and enhancer of GZMB. Blue arrows represent transcription start site. b qPCR analysis of chromatin-immunoprecipitated T cells showing interaction of TCF-1 with the IFNG promoter and GZMB enhancer region in primary T cells treated with or without FTY720 (n = 7). Data are shown as mean ± SEM. *p < 0.05, paired non-parametric t tests. c qPCR analysis of TCF7 expression in T cells treated with TCF7 shRNA (sh-TCF7) or negative control (sh-CTL). **p < 0.01, paired non-parametric t tests. d qPCR analysis of IFNG and GZMB expression in T cells treated with TCF7 shRNA or negative control. **p < 0.01, paired non-parametric t tests. e Luciferase activity in HEK293T cells transfected with plasmids encoding firefly luciferase downstream of the IFNG promoter region (IFNG-luc), Tbet, TCF-1, or empty vector control. f Luciferase activity in HEK293T cells transfected with plasmids encoding firefly luciferase downstream of the GZMB enhancer region (GZMB-luc), TCF-1 or empty vector control. RLU relative light units. Data are shown as mean ± SEM. * **p < 0.01, ***p < 0.001, unpaired non-parametric t tests