Interferon-stimulated genes—essential antiviral effectors implicated in resistance to Theiler’s virus-induced demyelinating disease

Table 1 Transcriptional changes of the type I IFN signaling pathway in TMEV- compared to mock-infected SJL/J mice

Gene symbol	14 dpi	42 dpi	98 dpi	196 dpi
Pattern recognition receptors
Eif2ak2 (Pkr)	1.69	3.04	3.73	2.18
Ifih1 (Mda5)	2.20	4.86	5.77	3.51
Tlr2	1.77	2.66	3.31	2.94
Tlr3	1.18	1.37	1.89	1.38
Tlr7	1.18	1.87	2.47	2.43
Tlr13	1.33	2.50	3.46	3.27
Interferon regulatory factors
Irf1	1.51	3.47	4.40	3.11
Irf3	1.07	1.06	1.09	1.18
Irf5	1.07	1.40	1.81	1.57
Irf7	2.66	10.12	11.42	4.46
Nfkb1	1.14	1.14	1.20	1.10
Type I interferons
Ifna1	1.04	−1.10	−1.06	−1.08
Ifnb1	1.02	1.07	−1.08	−1.11
Type I interferon receptors
Ifnar1	1.01	−1.02	1.14	1.15
Ifnar2	1.10	1.51	1.72	1.50
Signal transducers
Irf9	2.53	4.92	4.72	3.21
Jak1	1.01	1.02	−1.04	1.06
Socs1	1.01	1.31	1.48	1.14
Stat1	3.26	10.58	15.39	9.57
Stat2	1.39	2.49	3.30	2.03
Tyk2	1.15	1.12	1.32	1.19
Classical interferon stimulated genes
Isg15	3.23	11.58	15.71	6.59
Mx1	1.34	2.11	3.85	1.88
Mx2	1.24	1.61	1.76	1.31
Oas1a	2.71	7.92	11.07	6.73
Oas2	1.32	2.26	2.59	2.08
Oas3	1.01	1.59	1.75	1.23
Oasl1	1.61	2.81	3.65	1.99
Oasl2	6.70	25.78	29.16	12.93
Rnasel	−1.16	1.37	1.54	1.29
Major histocompatibility (MHC) genes class I/II
H2-D1 (MHC-Ia)	5.58	8.03	9.78	9.42
H2-K1 (MHC-Ia)	5.42	7.45	9.70	8.56
H2-M3 (MHC-Ib)	1.62	2.80	3.48	3.25
H2-Q5 (MHC-Ib)	2.46	9.75	13.88	10.7
H2-Aa (MHC-IIa)	5.49	29.30	37.55	29.10
H2-Eb1 (MHC-IIa)	5.11	20.11	28.18	21.00
H2-DMa (MHC-IIb)	1.31	2.93	4.23	3.22
H2-Oa (MHC-IIb)	1.25	2.78	4.39	2.79

Shown are fold changes at 14, 42, 98, and 196 dpi. Italic type indicates statistically significant up-regulation (P ≤ 0.05)
Legend: Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 4, H2-Aa H2 antigen A alpha, H2-D1 H2-D region locus 1, H2-DMa H2 antigen locus DMa, H2-Eb1 H2 antigen E beta 1, H2-K1 H2-K region locus 1, H2-M3 H2-M region locus 3, H2-Oa H2-O region alpha locus, H2-Q5 H2-Q region locus 5, Ifih interferon induced with helicase C domain, Ifit interferon-induced protein with tetratricopeptide repeats, Ifna interferon alpha, Ifnb interferon beta, Ifnar interferon (alpha and beta) receptor, Irf interferon regulatory factor, Isg interferon-stimulated protein, Jak Janus kinase, Mx Myxovirus (influenza virus) resistance, Nfkb nuclear factor of kappa light polypeptide gene enhancer in B cells, Oas 2′-5′ oligoadenylate synthetase, Rnasel Ribonuclease L (2′, 5′-oligoisoadenylate synthetase-dependent), Socs suppressor of cytokine signaling, Stat signal transducer and activator of transcription, Tlr Toll-like receptor, Tyk tyrosine kinase

ISSN: 1742-2094