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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Non-alcoholic fatty liver disease induces signs of Alzheimer’s disease (AD) in wild-type mice and accelerates pathological signs of AD in an AD model

Fig. 4

Long-term HFD decreased NeuN+ cells, Aβ plaques, and astrocytes in APP-Tg mice. a Representative images of cortex (left panel) or hippocampus (right panel) from APP-Tg mice fed with SD or HFD for 1 year, stained with anti-6E10 anti-β-amyloid antibody (green), anti-GFAP (red), and DAPI (blue). Scale bar = 200 μm. b Representative zoomed-in images of reactive astrocyte (red) around plaque (green) from the cortex of SD or HFD-fed APP-Tg mice. Scale bar = 50 μm. c Number of plaques from different regions of cortex (n = 10) or hippocampus (n = 10). * indicates p < 0.05 (two-tailed student’s t test, n = 2 mice/group, representative of two independent experiments). d Representative with SD or HFD for 2 months (top panel) or 1 year (bottom panel) stained with anti-NeuN (red), and anti-GFAP antibody (gold). Zoomed-in image was inserted as inlet. e Relative intensity of GFAP signal from d (n = 10). ** indicates p < 0.01 (two-tailed student’s t test, twice mice/group, representative of two independent experiments). Representative images (f) and quantitative analysis (g) of images from brain cortex of APP-Tg mice fed with SD or HFD stained with NeuN (red), or TUNEL (green). TUNEL/NeuN double positive cells were counted from ten different fields and quantified as a percentage of total cell count. *** indicates p < 0.001 (two-tailed student’s t test, two mice/group). h Representative image of brain sections from SD or HFD-fed APP-Tg mice stained with anti-NeuN antibody (red). Dotted lines indicate the outline of NeuN-positive signal in cerebral cortex. i Quantitative analysis of h *** indicates p < 0.001 (two-tailed student’s t test, two mice/group). j Cortical thickness of brains from SD or HFD-fed mice measured from ten different fields. ** indicates p < 0.001 (two-tailed student’s t test, two mice/group). k Quantitative analysis of h, showing intensity of NeuN-positive signal measured from ten different fields of dentate gyrus from mice fed SD or HFD. ** indicates p < 0.001 (two-tailed student’s t test, two mice/group)

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