Fig. 5

Long-term HFD induced Aβ plaque formation in brains of WT mice. a Representative images of brain sections from HFD-fed WT mice stained with anti-NeuN (red) and anti-6E10 (green). b Representative images of brain sections (cortex) from SD or HFD-fed WT mice double-stained with anti-NeuN (red) and TUNEL (green). c TUNEL/NeuN double positive cells were counted from ten different fields and quantified as a percentage of total cell count. d Representative image of brain sections from SD or HFD-fed WT mice stained with anti-NeuN antibody (dotted lines indicate the outline of NeuN-positive signal in the cerebral cortex. e Quantitative analysis of d, showing numbers of NeuN⁺ cells from ten fields of the brain. ** indicates p < 0.01 (two-tailed student’s t test, two mice/group). f Comparison of cortical thickness of brains from SD or HFD-fed mice. Thickness of cortex was measured from ten different fields. ** indicates p < 0.01 (two-tailed student’s t test, two mice/group). g Quantitative analysis of d, capturing the intensity of NeuN-positive signal measured from ten different fields of the dentate gyrus from brains of mice fed with SD or HFD. ** indicates p < 0.01 (two-tailed student’s t test, two mice/group). These are representative findings from two independent experiments