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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Non-alcoholic fatty liver disease induces signs of Alzheimer’s disease (AD) in wild-type mice and accelerates pathological signs of AD in an AD model

Fig. 6

Long-term HFD induced advanced signs of AD in WT and APP-Tg mice. a Representative images of hippocampus from WT and APP-Tg mice fed with SD or HFD for 1 year, stained with anti-phosphorylated-Tau (S396) antibody. b, c Western blot analysis of brain homogenate of WT or APP-Tg mice fed with SD or HFD for 1 year (b). Phosphorylated-Tau was detected with S396 or AT8 antibody and total Tau protein was detected with anti-Tau antibody. GAPDH was used as loading control. Intensity of phospho-Tau was normalized by that of Tau protein which was further normalized by intensity of GAPDH and depicted in graph (c). ** indicates p < 0.01. (two-tailed student’s t test, n = 2). d Representative brain sections from WT mice fed SD or HFD and stained with anti-CD31 (red) and anti-β- amyloid antibody, 6E10 (green) to assess the degree of cerebral amyloid angiopathy (CAA) and vasculopathy. The top three panels depict SD controls, the bottom three panels show HFD-induced CAA. e Representative brain images from APP-Tg mice double-stained with antibodies to β-amyloid (6E10) and CD31 (vasculature) for SD (top three panels) or HFD (bottom three panels) to evaluate CAA. f Quantification of fragmented vessels taken over multiple microscopic fields throughout the brains of WT and APP-Tg mice fed with SD or HFD. *** indicates p < 0.001 (Two-tailed student’s t test, ten fields/mouse, n = 2 mice/group)

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