Fig. 7
Long-term HFD decreased LRP1 and maintained chronic inflammation in brains of WT and APP-Tg mice mRNA expression of LRP1 (a) or ApoE (b) was analyzed in the brains of mice fed SD or HFD for 2 months or 1 year by quantitative real-time PCR. * and ** indicate p < 0.05 and p < 0.01, respectively (two-tailed student’s t test, n = 2 mice/group performed in triplicates, representative of two independent experiments). c Splenocytes from mice fed SD or HFD for 1 year were stimulated with PMA-ionomycin for 3 days, supernatants were collected and pro-inflammatory cytokines (TNF-α, IL-6, IL-17) were quantified by ELISA. *, **, and *** indicate p < 0.05, p < 0.01, and p < 0.001, respectively (two-tailed student’s t test, n = 2 mice/group performed in triplicates, representative of two independent experiments). d mRNA expression level of pro-inflammatory cytokines (TNF-α, IL-6, IL17) was analyzed in brain samples from mice fed HFD or SD for 1 year by quantitative real-time PCR * and ** indicate p < 0.05 and p < 0.01, respectively (two-tailed student’s t test, n = 2 mice/group performed in triplicates, representative of two independent experiments)