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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Post-CNS-inflammation expression of CXCL12 promotes the endogenous myelin/neuronal repair capacity following spontaneous recovery from multiple sclerosis-like disease

Fig. 4

Numbers of CXCL12+ OPCs are elevated in the spinal cord of mice as EAE progresses and are further increased following spontaneous recovery from the disease. a Representative images of the spinal cord tissue sections from naïve healthy mice and from mice with clinical EAE (at the peak) co-immunostained for OPCs (NG2+, green) and CXCL12 (red). b Quantitative analysis of OPCs (NG2+) co-expressing CXCL12 (NG2+ CXCL12+) in the spinal cord of mice with EAE compared to naïve mice (*p < 10−7: data are from five consecutive sections per mouse, n = 3 mice/group). ce NG2+ OPCs and CXCL12 colocalize in the spinal cord sections of mice with EAE. c Confocal image of two OPCs (NG2+, green): one is CXCL12 positive (red) and the other is CXCL12 negative, in a spinal cord section (L4) from a mouse with clinical EAE. d The left image is a higher magnification of the boxed area in c. The right panel represents colocalization analysis of serial levels (each 0.5 μm). Numbers in the table represent weighted colocalization coefficient (WCC) values of NG2+ cell that do not express CXCL12 (marked 1) compared to NG2+ cell that co-express CXCL12 (marked 2). WCC values were measured with Zeiss LSM image examiner software. In the table, values for CXCL12 (red) denote the colocalization coefficient from the red channel colocalized with the green channel, and the values for NG2 (green) denote the colocalization coefficient from the green channel colocalized with the red channel for OPCs marked 1 and 2. e Quantitative analysis of the mean WCC values of NG2+ OPCs co-expressing CXCL12 in mice with EAE compared to naïve mice (*p < 0.001, **p = 0.002). Data are from five consecutive sections from each mouse, n = 3 mice/group. Error bars represent mean ± SEM from two independent experiments. Nuclei were visualized by DAPI staining (blue). Scale bars: a 20 μm; c, d 10 μm. Arrowheads indicate OPCs (NG2+) that co-express CXCL12

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