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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Post-CNS-inflammation expression of CXCL12 promotes the endogenous myelin/neuronal repair capacity following spontaneous recovery from multiple sclerosis-like disease

Fig. 5

Numbers of CXCL12+ OPCs are elevated in the CC of mice with onset and the progression of clinical EAE and further increase following spontaneous recovery from the disease. a Representative images of the CC of mice with EAE clinical episodes or following spontaneous recovery; sections were co-immunostained for OPCs (NG2+, green) and CXCL12 (red). b Orthogonal view of an OPC (NG2+, green) in the CC of EAE-recovered mouse that express CXCL12 (red). The middle image is a higher magnification of the boxed area in the left image. Colocalization of NG2 (green) and CXCL12 (red) was confirmed by optical dissection and orthogonal reconstruction of the confocal image (right image). c Quantitative analysis of the OPCs (NG2+) and OPCs that co-express CXCL12 (NG2+CXCL12+) in the CC of mice with EAE clinical episodes or following spontaneous recovery (*p < 0.00001 and **p < 0.0003 compared to naive; ***p < 0.0005, recovered compared to peak, n = 4 mice/group). Data are from five consecutive sections from each mouse. Data represent mean ± SEM from three independent experiments. Nuclei were visualized by DAPI counterstaining (blue). Scale bar: a 50 μm; left image in b, 50 μm; boxed area in b, 5 μm. Arrowheads indicate OPCs (NG2+) co-expressing CXCL12

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