Fig. 8

Intrathecal injections of LY303870, anakinra, and anti-NGF at 4 and 16 weeks post fracture reversed allodynia and unweighting. LY303870 (10 μl at 20 μg/μl, 30 min prior to testing), anakinra (10 μl at 10 μg/μl, 1 h prior to testing), and anti-NGF (10 μl at 1.24 μg/μl, 1 h prior to testing) were administered via intrathecal injection. a At 4 weeks, fracture induced allodynia and unweighting. Treatments with LY303870, anakinra, and anti-NGF reduced fracture-induced allodynia by 52, 84, and 62 %, respectively. b The same treatments reduced unweighting by 58, 60, and 60 %, respectively. c At 16 weeks, allodynia and unweighting were still significant. Treatments with LY303870, anakinra, and anti-NGF reduced fracture-induced allodynia by 54, 79, and 67 %, respectively. d The same treatments at 16 weeks post fracture reduced unweighting by 88, 61, and 67 %, respectively. Measurements for allodynia and unweighting were carried out and calculated as described for Fig. 1. Data are expressed as mean values ± standard error (SE) and analyzed using one-way ANOVA followed by Neuman-Keuls multiple comparison test to compare control and fracture at 4 weeks cohorts (control, n = 6; FX- 4 WK, n = 7; FX- 4 WK + LY303870, n = 7; FX- 4 WK + anakinra, n = 8; FX- 4 WK + anti-NGF, n = 8) as well as fracture with the treatment versus fracture cohorts at 16 weeks (control, n = 6; FX- 16 WK, n = 8; FX- 16 WK + LY303870, n = 8; FX- 16 WK + anakinra, n = 6; FX- 16 WK + anti-NGF, n = 8). *P < 0.05, **P < 0.01, and ***P < 0.001 versus control; ^# P < 0.05, ^## P < 0.01, and ^### P < 0.001 versus fracture at 4 or 16 weeks