Fig. 4

CXCL5 upregulation in the white matter after LPS-sensitized HI. a Western blots of the white matter revealed significant increases in CXCL5 levels at 6 and 24 h after LPS + HI (n = 4 in each study group). b Immunohistochemical analyses revealed that the LPS + HI group had significantly increased CXCL5 immunoreactivity in the white matter at 6 h and, particularly, 24 h after HI compared with the control group (n = 6 in each study group). Scale bar = 50 μm. Values are means ± SEMs. c Immunofluorescence analysis of the white matter 24 h after LPS + HI revealed that CXCL5 was upregulated mainly in activated microglia (ED1) and vascular endothelial cells (RECA) and not in astrocytes (GFAP) or preoligodendrocytes (O4). Scale bar = 50 μm. *p < 0.05, **p < 0.01