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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Exosomes derived from atorvastatin-modified bone marrow dendritic cells ameliorate experimental autoimmune myasthenia gravis by up-regulated levels of IDO/Treg and partly dependent on FasL/Fas pathway

Fig. 3

Distribution of Dex after injection into the EAMG rats and effects of statin-Dex injection on endogenous DCs. The PKH26-labeled (red) Dex were detected in the spleen, thymus, and popliteal and inguinal lymph nodes on days 1 and 3 after injection (a, original magnification ×100). The expression of CD80, CD86, and MHC class II on endogenous DCs on days 1 and 3 after injection were examined by FACS. The results showed that endogenous DCs from EAMG rats of statin-Dex group expressed lower level of CD80 on day 1 after injection and lower levels of CD80, CD86, and MHC class II on day 3 after injection when compared with those from control-Dex group. There were no significant differences for the levels of CD86 and MHC class II expressed on endogenous DCs from EAMG rats on day 1 after injection between statin-Dex group and control-Dex group (b). To further investigate the mechanism of exogenous Dex injection on endogenous DCs, spleen-derived DCs from ongoing EAMG rats were cultured with PKH26-labeled (red) statin-Dex and control-Dex in vitro, respectively. The percentages of PKH26-labeled endogenous DCs were examined by flow cytometry (c). Meanwhile, these DCs were labeled with mouse anti-rat OX62 antibody and FITC-conjugated anti-mouse IgG antibody (green). Then, the labeled DCs were examined by fluorescence microscopy. Both statin-Dex and control-Dex could be internalized by or fused with spleen-derived DCs in vitro (red stands for Dex, green stands for DCs, and yellow refers to overlap of red and green) (The diameter of spleen-derived DCs from rats is about 10–20 μm) (d, original magnification ×400). The results are expressed as mean ± SD (n = 5 rats per group) (*p < 0.05 and ***p < 0.001)

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