Fig. 5

Increase in CCL3 mRNA level is correlated with a decrease in amyloid load in ASC+/− 5xFAD 8-month-old mice. Expression of CCL3 was determined by qRT-PCR on cDNA prepared from the hippocampus of 8-month-old WT and 5xFAD ASC mice. Statistical differences were determined using an ANOVA analysis and post Bonferroni multiple comparison test. ***P < 0.001 vs all others and ⁺⁺⁺ P < 0.001 (a) (5 F− A+/+, 10 F+ A+/+, and 6 F+ A+/−). Thioflavin S staining was performed on sections from the hippocampus of 8-month-old WT and 5xFAD ASC mice (b) (7 F+ A+/+ and 4 F+ A+/−). Amyloid plaques were quantified using image J and are represented as the mean % of total area ± SEM. Significance was assessed using an ANOVA analysis and post Bonferroni multiple comparison test. *P < 0.05 vs all others (c)