Fig. 4

Effects of U0126, apocynin, or p47phox knockout on cytosolic and mitochondrial oxidative burdens after MA. Changes in cytosolic and mitochondrial reactive oxygen species (ROS) formation (a) and mitochondrial MnSOD expression (c) after MA treatment and effects of U0126, apocynin, or p47phox knockout on ROS (b) and MnSOD expression (d) 2 h after MA (35 mg/kg, i.p.). WT wild-type mice. p47 KO p47phox knockout mice, Sal saline, U U0126 (2 μg, i.c.v.), Apo apocynin (50 mg/kg, i.p.), V or Veh vehicle [10 % (v/v) DMSO] for U0126 or apocynin. Each value is the mean ± S.E.M. of six animals. ^* P < 0.05, ^** P < 0.01 vs. saline or vehicle/WT with saline. ^& P < 0.001 vs. saline. ^# P < 0.05, ^## P < 0.01 vs. vehicle/WT with MA [one-way ANOVA (a and c) or three-way ANOVA (b and d) was followed by Fisher’s LSD pairwise comparisons]