Fig. 6

Effects of U0126, apocynin, or p47phox knockout on Iba-1 expression after MA. Changes in Iba-1 expression after MA treatment (a) and effects of U0126, apocynin, or p47phox knockout on Iba-1 expression (b) and microglial activation as labeled by Iba-1 (c, d) 1 day after MA (35 mg/kg, i.p.). WT wild-type mice, p47 KO p47phox knockout mice, Sal saline, U or U0126 U0126 (2 μg, i.c.v.), Apo apocynin (50 mg/kg, i.p.), V or Veh vehicle [10 % (v/v) DMSO] for U0126 or apocynin. Each value is the mean ± S.E.M. of six animals. ^* P < 0.05, ^** P < 0.01 vs. saline or vehicle/WT with saline. ^# P < 0.05, ^## P < 0.01 vs. vehicle/WT with MA [one-way ANOVA (a) or three-way-ANOVA (b, d) was followed by Fisher’s LSD pairwise comparisons]. Scale bar = 100 μm