Fig. 8

Effects of U0126, apocynin, or p47phox knockout on pro-apoptotic changes after MA. Effects of U0126, apocynin, or p47phox gene knockout on TUNEL-positive cells (a), cytosolic release of cytochrome c (b), and cleaved caspase 3 expression (c) 1 day after MA (35 mg/kg, i.p.) in the Taconic ICR mice. p47 SO or p47phox SO p47phox sense oligonucleotide, p47 ASO or p47phox ASO p47phox antisense oligonucleotide, Sal saline, U or U0126 U0126 (2 μg, i.c.v.), Apo apocynin (50 mg/kg, i.p.), V or Veh vehicle [10 % (v/v) DMSO] for U0126 or apocynin. Each value is the mean ± SEM of six animals. ^** P < 0.01 vs. respective saline-group. ^# P < 0.05, ^## P < 0.01 vs. vehicle/p47phox SO with MA or vehicle/vehicle with MA (three-way ANOVA was followed by Fisher’s LSD pairwise comparisons). Scale bar = 100 μm