Fig. 9

Effects of U0126, apocynin, or p47phox knockout on dopaminergic impairments after MA. Changes in TH expression (a), dopamine level (d), and dopamine turnover rate (f) after MA treatment and effects of U0126, apocynin, or p47phox gene knockout on TH expression (b), TH-immunoreactivity (c), dopamine level (e), and dopamine turnover rate (g). WT wild-type mice, p47 KO p47phox knockout mice, Sal saline, U or U0126 U0126 (2 μg, i.c.v.), Apo apocynin (50 mg/kg, i.p.), V or Veh vehicle [10 % (v/v) DMSO] for U0126 or apocynin. Each value is the mean ± S.E.M. of six animals. ^* P < 0.05, ^** P < 0.01 vs. saline or vehicle/WT with saline. ^# P < 0.05 vs. vehicle/WT with MA [one-way ANOVA (a, d, and f) or three-way ANOVA (b, c, e, and g) was followed by Fisher’s LSD pairwise comparisons]. Scale bar = 1 mm