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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta

Fig. 3

Response of IMG cells to pro- and anti-inflammatory stimuli. IMG cells were treated for either 8 or 24 h with LPS (10 ng/mL), interferon gamma (IFNγ) (10 ng/mL), IL-1β (10 ng/mL), TNF-α (5 ng/mL), IL-4 (10 ng/mL), IL-13 (10 ng/mL), IL-6 (10 ng/mL), or TGF-β (50 ng/mL). iNOS, TNF-α, and IL-1β message levels were used as markers for the microglial pro-inflammatory phenotype (a), whereas Arg-1, Mgl1, and Ym1 were used as markers for an anti-inflammatory phenotype (b). Quantitative PCR was used to assess the changes in message levels of the aforementioned genes relative to the internal control 36B4. Average of biological duplicates are shown with similar results obtained in at least two different experiments

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