Fig. 7

IMG cells retain phagocytic properties inherent in microglial cells. Phagocytosis of 1-μm fluorescent yellow-green latex beads (YG beads) by IMG (a) or BV-2 (b) cells as analyzed by flow cytometry. Cells were incubated with the YG beads for 1 h at either 4 °C (open trace) or 37 °C (gray fill) prior to analysis by flow cytometry. Each peak represents the IMG population which has ingested either 0, 1, 2, 3, or 4 beads, respectively. Each graph (a, b) is a representation of data from at least three biological replicates. c Percentage of cells which acquired ≥1 YG beads as analyzed by flow cytometry. d Kinetic analysis of oligomeric FITC-Aβ uptake by IMG. IMG cells were incubated with FITC-Aβ (1 μM) for the indicated times at 37 °C. IMG-associated FITC fluorescence was measured using a plate reader. e Subcellular localization of internalized FITC-Aβ was assessed using fluorescence microscopy. IMG cells were incubated for 1 h with FITC-Aβ (green) prior to being processed for microscopy. Phagolysosomes were identified using LAMP1 antibody (red). Areas of co-localization (white) were determined using ImageJ software. A paired t test was used to determine the significance of the data (n.s. is not significant). Data are represented as means ± s.d. (n = 3, experimental replicates)