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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Complement component C3a plays a critical role in endothelial activation and leukocyte recruitment into the brain

Fig. 3

Effect of C3 deficiency on the expression of endothelial adhesion molecules and the BBB permeability changes in the brain. WT C57BL/6J mice and C3−/− mice received i.c.v. injections of LPS; 4 h later, mRNA expression in brain tissue was quantified using real-time PCR. a C3 deficiency resulted in the downregulation of brain P-selectin, E-selectin, ICAM-1, and VCAM-1 mRNA expression (n ≥ 8). b VCAM-1 and E-selectin protein levels were measured through Western blotting analysis; the ratio of VCAM-1 and E-selectin to β-actin was determined through densitometry (n = 5). c To assess BBB permeability, mouse brain homogenates were examined for albumin infiltration using Western blotting analysis (n = 6). Albumin levels in the brain proteins were measured at 4, 12, and 24 h after LPS i.c.v. The results are shown as the means ± SEM; *P < 0.05

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