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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: HSP60 plays a regulatory role in IL-1β-induced microglial inflammation via TLR4-p38 MAPK axis

Fig. 1

IL-1β induces inflammation both in vitro and in vivo. a Left panel shows the representative western blot images of iNOS and COX2 from N9 cell lysates at 3, 6, and 12 h after 5 ng/ml of IL-1β treatment. Right panel shows the bar diagrams which represent mean fold change in the levels of iNOS and COX2 after IL-1β treatment with respect to control. b Bar diagrams represent the mean fold change after CBA analysis of pro-inflammatory cytokines, i.e., TNF-α, MCP-1, and IL-6 after IL-1β treatment at 3, 6, and 12 h. c Left panel shows the representative western blot images of iNOS and COX2 from P10 BALB/c mice brain after IL-1β treatment (10 ng/g of body weight, intraperitoneally injected) for different time periods (1, 3, and 5 days). Right panel shows the bar diagrams which represent mean fold change in the level of respective proteins in comparison to control at different time points. One hundred microgram of the protein was loaded for western blot (a and c) and the levels of iNOS and COX2 were normalized with β-actin. d CBA analysis of pro-inflammatory cytokines (TNF-α, MCP-1, and IL-6) at different time points of IL-1β treatment. Data represent mean ± SD from three different sets of experiments. *p < 0.05, **p < 0.01 in comparison to untreated control condition

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