Fig. 8

HSP60 regulates phosphorylation of MAPK effector molecules ERK1/2, JNK, and p38. a Upper panel depicts the western blot analysis of phospho- and total ERK1/2, JNK, and p38 in the N9 cells transfected with HSP60 esiRNA (at 6pM dose) or scrambled esiRNA (6pM). Lower panel shows the bar diagram which represents mean fold changes in the levels of phosphorylated forms of aforementioned proteins with respect to their total proteins. b Overexpression of HSP60 cDNA clone in microglial cells leads to increase in phosphorylation of all the three MAPKs at different doses of HSP60 cDNA clone. One hundred microgram protein was loaded for western blots, and β-actin was used as a loading control. Representative of three independent experiments is shown here. Graphs in lower panel represent mean fold change in the level of phosphorylation of MAPKs. The levels of phosphorylated proteins were normalized to their total proteins, respectively. Data represented are mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 in comparison to control values and ^# p < 0.01 with respect to IL-1β-treated values