Fig. 1

IPS-1 signaling controls LGTV replication in the CNS. a, b Survival of WT and IPS-1 ^−/− mice. Mice were inoculated with 10⁴ FFU of LGTV (WT n = 10, IPS-1 ^−/− n = 10) or TBEV strain Hypr 71 (WT n = 10, IPS-1 ^−/− n = 5) via the intraperitoneal route and monitored for mortality for 21 days. Survival differences were tested for statistical significance by the log-rank test. c Viral burden in serum (n = 5). Serum were harvested various days post intraperitoneal infection of LGTV (10⁴ FFU). Viral RNA in the serum was quantified by real-time RT-PCR detecting the LGTV NS3 and compared with a standard curve derived from control serum samples spiked with LGTV. Number sign means not detectable; dotted line represents the detection limit. d–g Viral burden in different organs after intraperitoneal infection of LGTV (10⁴ FFU, n = 5–6) measured by real-time qPCR detecting LGTV NS3 gene (detection limit 10 copies) and normalized to intracellular GAPDH levels. d Spleen. e Lung. f Spinal cord. g Brain. Asterisks indicate statistical significance calculated by Mann-Whitney test, **p < 0.01, *p < 0.05. au arbitrary units