Fig. 5

IPS-1 signaling controls local antiviral response within CNS. a Survival analysis of WT and IPS-1 ^−/− mice. Mice (n = 9–10) were inoculated with 10 or 100 FFU of LGTV via the intracranial route and monitored for mortality for 21 days. Survival differences were tested for statistical significance by the log-rank test, * p < 0.05. b–c WT and IPS-1 ^−/− mice were infected with LGTV intraperitoneally (10⁴ FFU) or intracranially (10² FFU) and different regions of the brain, olfactory bulb, cerebrum, cerebellum and brain stem, were harvested 7 dpi and 5 dpi, respectively. Viral burden (b) were quantitated by real-time qPCR detecting the 3′ NCR (detection limit 10⁴ copies) n = 10 per experiment. IFN-β fold induction (c) after infection was calculated by measuring IFN-β levels in uninfected and infected brain parts by real-time RT-PCR, setting the IFN-β level in uninfected control samples to one and calculate the fold induction of IFN-β RNA transcript from infected brain parts. Asterisks indicates statistical significance calculated by Mann-Whitney test, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. ns not significant, au arbitrary units