Fig. 6

Effect of treatment with NaSH or STS on SH-SY5Y cell viability changes induced by LPS/IFNγ-activated THP-1 cell conditioned medium (CM) as followed by MTT and lactate LDH release assays (protocol 1). a 2 h pre-incubation, b 4 h pre-incubation, c 8 h pre-incubation, and d 12 h pre-incubation. Upper panel: MTT assay data and lower panel: LDH release assay data. Values are mean ± SEM, n = 4. Two-way ANOVA was carried out to test significance. Multiple comparisons were followed with post hoc Bonferroni tests. ⁺ P < 0.01 for LPS/IFNγ-activated groups compared with CON groups in the same concentrations, *P < 0.01 for NaSH- or STS-treated groups compared with LPS/IFNγ-activated groups, ^# P < 0.01 for STS-treated groups compared with NaSH roups in the same concentrations (a–d). Note that both compounds attenuated a reduction of SH-SY5Y cell viability induced by LPS/IFNγ-activated THP-1 cell CM in a concentration-dependent and pre-incubation time-dependent manner