Fig. 8

Effect of pre-treatment with NaSH or STS for 8 h on SH-SY5Y cell viability changes induced by a LPS/IFNγ-activated microglial CM and b IFNγ-activated astrocytic CM as followed by MTT and LDH release assays (protocol 1). Upper panel: MTT assay data and lower panel: LDH release assay data. Values are mean ± SEM, n = 4. Two-way ANOVA was carried out to test significance. Multiple comparisons were followed with post hoc Bonferroni tests. ⁺ P < 0.01 for LPS/IFNγ- (a) or IFNγ-activated (b) groups compared with CON groups in each condition, *P < 0.01 for NaSH- or STS-treated groups compared with LPS/IFNγ- or IFNγ-activated groups, ^# P < 0.01 for STS groups compared with NaSH groups in each condition. Note that both compounds attenuated a reduction of SH-SY5Y cell viability induced by LPS/IFNγ-activated THP-1 cell CM in a concentration-dependent manner