Fig. 9

Effect of pre-treatment with NaSH or STS for 8 h on levels of phospho-P38 MAPK and phospho-P65-NFκB in LPS/IFNγ-activated human microglia a (left panel) and IFNγ-activated astrocytes a (right panel). Cell extracts were prepared and the proteins separated by SDS-PAGE. Representative blots are shown in a and quantitative results in b. To ensure equal loading, the densitometric value of each band was normalized to the corresponding band for α-tubulin. Values are mean ± SEM, n = 3. One-way ANOVA was carried out to examine the significance of differences. ⁺ P < 0.01 for LPS/IFNγ- or IFNγ-activated groups compared with CON groups in each condition, *P < 0.01 for NaSH- or STS-treated groups compared with LPS/IFNγ- or IFNγ-activated groups, ^# P < 0.01 for STS-treated groups compared with NaSH groups in each conditions. Note that both compounds attenuated the levels of phospho-P38 MAPK and phospho-P65-NFκB in both cells