Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Angiotensin-converting enzyme 2 activator diminazene aceturate prevents lipopolysaccharide-induced inflammation by inhibiting MAPK and NF-κB pathways in human retinal pigment epithelium

Fig. 1

The optimum concentration and incubation time of DIZE in cultured ARPE-19 cell line. a The cell viability was measured by CCK-8 assay. ARPE-19 cells were treated with 0.01, 0.1, 1, 10, and 100 μg/mL of DIZE for 3, 6, and 12 h and then stimulated with LPS for 24 h. (***p < 0.001, ### p < 0.001, +++ p < 0.001, n = 6, “asterisk” “number sign” “plus sign” indicated pre-incubated with 100 μg/mL of DIZE for 3, 6, and 12 h versus time-matched untreated cells, respectively). b ARPE-19 cells were pre-treated with 10 μg/mL of DIZE for 3, 6, and 12 h, following stimulated with LPS for 24 h. The mRNA expression of IL-6, IL-8, and MCP-1 in the DIZE-treated groups were compared with the LPS group by real-time PCR. All data are expressed as mean ± SEM. (***p < 0.001, **p < 0.01, *p < 0.05, n = 4 ~ 6)

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094