Fig. 12

Effect of ACE2-siRNA on the phosphorylation of MAPKs and the activation of NF-κB in ARPE-19. The protein levels of p-p38, p-JNK, p-ERK1/2, p38, JNK, ERK1/2 (a) and p-IκB-α (b) were determined by Western blotting. The band intensity of p-p38, p-JNK, and p-ERK1/2 was normalized to p38, JNK, and ERK1/2, respectively. The band intensity of p-IκB-α was normalized to β-actin. DIZE reduced the phosphorylation of p38, JNK, and ERK1/2 and IκB-α induced by LPS, while ACE2-siRNA abolished DIZE’s effect. All data are expressed as mean ± SEM (***p < 0.001, **p < 0.01, *p < 0.05, n = 3 ~ 4)