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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Angiotensin-converting enzyme 2 activator diminazene aceturate prevents lipopolysaccharide-induced inflammation by inhibiting MAPK and NF-κB pathways in human retinal pigment epithelium

Fig. 4

The mRNA and protein expressions of pro-inflammatory cytokines in DIZE pre-treated hRPE and ARPE-19 cells followed by LPS stimulation. Real-time PCR and ELISA were conducted to determine the production of IL-6, IL-8, and MCP-1 in ARPE-19 cells (a) as well as hRPE (b). Exposure to LPS for 24 h caused a significant overproduction of IL-6, IL-8, and MCP-1 in the ARPE-19 cells and hRPE. Whereas overproduction of the cytokines was markedly inhibited by DIZE. IL-6 and IL-8 protein levels were undetectable in the DIZE group. All data are expressed as mean ± SEM. (***p < 0.001, **p < 0.01, *p < 0.05, n = 4)

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Journal of Neuroinflammation

ISSN: 1742-2094