Fig. 3From: RGS10 deficiency ameliorates the severity of disease in experimental autoimmune encephalomyelitisRGS10-null naĂŻve CD4+ T cells displayed intact mitogen-mediated activation and polarization into Th1 or Th17. a CD4+ T cells were isolated from spleens of WT and RGS10-null mice. Cells were treated with PMA (20Â ng/ml)/ionomycin (1Â ÎŒM) or anti-CD3/CD28 (5Â ÎŒg/ml) in vitro for 72Â h. Proliferation was measured by MTS incorporation assay and (b) cytokine production was measured by multiplexed immunoassays (MSD). *, p < 0.05, ***, p < 0.001, two-way ANOVA. c Splenic naive T cells (CD4+ CD25â) were isolated from WT and RGS10-null mice. Cells were differentiated in vitro under Th1 (top panels) or Th17 (bottom panels) polarization conditions as described in the âMethodsâ section. PMA/ionomycin and protein transport inhibitors were added for the last 5Â h of the culture period. Cells were then stained with mAbs to surface markers and intracellular cytokines and analyzed by flow cytometry. CD4+ T lymphocytes were evaluated for IFNÎł and IL-17A expression. Representative FACS plots indicate the percentage of CD4+ T cells that stained positive for IFNÎł or IL-17A. Two independent experiments with similar results were performed (nâ=â2â3 mice in each experiment)Back to article page