Fig. 5

RGS10-null mice with symptomatic EAE have fewer leukocytes in the CNS. Mononuclear cells from the spinal cord were isolated from MOG_35–55-immunized WT (n = 3–4) and RGS10-null (n = 4) mice at day 15 p.i. as described in the “Methods” section. Cells were then stained with mAbs to surface markers and/or intracellular cytokines and analyzed by flow cytometry. a Plot shows the CD3+ T cells, CD19+ B cells, Ly6G+ neutrophils, and CD11b+ myeloid cells among CD45+ gated cells in the spinal cord. *, p < 0.05, Student's t test. b Plot shows the CD45^low (microglia) and CD45^high (macrophages or activated microglia) cells among Ly6G-CD11b+ MHCII+ gated cells. *, p < 0.05, two-way ANOVA. c Plot shows the T-bet+ (Th1) and RORγt+ (Th17) cells among CD3+ CD4+ gated cells. *, p < 0.05, **, p < 0.01, two-way ANOVA