Fig. 2From: Suppression of LPS-induced tau hyperphosphorylation by serum amyloid AImmunofluorescence staining of Saa3 in the CA region of the hippocampus. Frozen sections from the left hemisphere of mice were stained for Saa3 protein using a rabbit anti-Saa3 antibody and Alexa Fluor 488-conjugated anti-rabbit IgG (green fluorescence). The sections were subsequently stained for neurons (anti-MAP2; a), microglia (anti-CD11b; b), or astrocytes (anti-GFAP; c), all in red fluorescence (see the “Methods” section for detail). Cell nuclei were stained with DAPI (blue fluorescence). Immunofluorescence was detected using a confocal laser-scanning microscopy. Images shown are representative of multiple experiments (n = 4 for each group; scale bar, 50 μm). Arrowheads in the merged image mark the positions of the double-stained cells. d quantification of the tissue expression level of Saa3, CD11b, and GFAP after LPS stimulation (15 mg/kg, 24 h). The results are expressed as the means ± SEM from at least three mice per group, each in duplicates or triplicates. *p < 0.05Back to article page