Fig. 6

S1PR modulation selectively attenuates TLR4, but not TNFR/IL17R, mediated increase in the levels of IL6 in astrocytes. a Astrocytes or microglia were serum starved for 4 h, pre-treated with indicated concentrations of BAF312 and then treated with LPS (1–100 ng/ml) or TNFα/IL17. b Mouse astrocytes treated with LPS increased levels of IL6, which was modestly attenuated by BAF312 (n = 7). c Human astrocytes treated with LPS did not increase levels of IL6 (data not shown). In agreement with our previous studies, IL17/TNFα increased levels of IL6; however, these were not inhibited by BAF312 or FTY720 (n = 4). d, e Mouse microglia treated with LPS (1–100 ng/ml) increased IL6 levels in a concentration-dependent manner. pFTY720 and BAF312 moderately attenuated the LPS-induced increases in IL6. Data presented as ±SEM (n = 3–7), one-way ANOVA and Newman-Keuls multiple comparison post-test *p < 0.05, **p < 0.01, ***p < 0.001