Fig. 7

BAF312 attenuates LPC-induced demyelination and IL-6 levels in mouse organotypic cerebellar slice cultures. A Experimental timeline is shown, as previously described [9]. Organotypic cerebellar slice cultures were treated with LPS (0.5 mg/ml) for 18 h in the presence or absence of BAF312 and then treated with BAF213 alone for an additional 30 h (totalling 48 h) after which slices were processed for immunostaining. Bi Representative images show BAF312 attenuates LPC-induced demyelination as measured by MBP immunostaining, with limited effects on NFH immuoreactivity. Bii, Biii Data analysis of three separate experiments, demonstrating effects of BAF312 on LPC-induced changes in MBP and NFH immunostaining. C Organotypic cerebellar slice cultures were treated with LPS (0.5 mg/ml) for 18 h in the presence or absence of BAF312, and the media was processed for ELISA. Treatment with BAF312 attenuated the levels of IL6. Data presented as ±SEM (n = 3–4), one-way ANOVA and Newman-Keuls multiple comparison post-test. *p < 0.05, **p < 0.01, #p < 0.05 compared to LPC