Fig. 2

Primary rBEC cultures on Col I-coated Transwell® inserts were subjected to normoxia, IL-1β treatment for 4 h, 2.5 h OGD and 4 h reoxygenation (OGD + R), 2.5 h OGD, and 4 h reoxygenation in the presence of IL-1β (OGD + R + IL-1). Immunocytochemical images for occludin (a) were analysed with ImageJ to measure cell circularity (b), total intensity of occludin fluorescence (c), and ratio of mean cytoplasmic fluorescence intensity to mean membranous occludin fluorescence intensity (d). Data are shown as mean ± standard deviation from three independent experiments carried out on separate cultures. Data were statistically analysed by one-way ANOVA and Bonferroni’s post hoc tests to compare all data sets. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. normoxia. The scale bar in a represents 100 μm