Fig. 4

Primary rBEC cultures on Col I-coated Transwell® inserts were subjected to normoxia, or OGD (2.5 h) with (2.5 + 2, OGD + R) or without (2.5 + 0) reoxygenation for 2 h, and in the absence or the presence of IL-1β for 2 h. Total RNA was extracted, and RT-PCR was carried out for lmα4 (a), lmα5 (b), lmβ1 (c), lmγ1 (d), fn (e), col4 (f), and the housekeeping gene Rpl13a (ribosomal protein L13A). Products were resolved on an ethidium bromide-containing 1.5 % (w/v) agarose gel by electrophoresis and then quantified using GE healthcare ImageQuant™. Data are shown as mean ± standard deviation from three independent experiments carried out on separate cultures. Data were statistically analysed by one-way ANOVA and Bonferroni’s post hoc tests to compare all data sets. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. normoxia