Fig. 5

Primary rBECs were seeded onto tissue culture plastic pre-coated with BSA (TcP) or increasing concentrations of Col I, Col IV, FN, LM8, or LM10 for 90 min, and cell attachment was measured and expressed as percentage of cell number seeded (a). Primary rBECs were seeded onto wells coated with 100 nM BSA (TcP), Col I, Col IV, FN, LM8, or LM10 for 120 min, and cell spreading was measured and expressed as percentage of cell number seeded (b). Primary rBECs seeded onto Col I-, Col IV-, FN-, or LM10-coated (100 nM) Transwell® inserts were cultured for 1 to 4 days, after which TEER was measured (c). Data are shown as mean ± standard deviation from three independent experiments carried out on separate cultures. Data were statistically analysed by one-way ANOVA and Bonferroni’s post hoc tests to compare all data sets. For b, **p < 0.01 and ***p < 0.001 vs. TcP; ^††† p < 0.001 vs. Col I. For c, *p < 0.05 vs. Col I