Fig. 6

Primary rBECs seeded onto Col I-, Col IV-, FN-, or LM10-coated (100 nM) Transwell® inserts were cultured for 4 days, after which cultures were treated with vehicle or IL-1β for 4 h. TEER measurements were carried out at the end of each treatment (a). Immunocytochemical images for occludin (b) were analysed with ImageJ to measure total intensity of occludin fluorescence (c), ratio of mean cytoplasmic fluorescence intensity to mean membranous occludin fluorescence intensity (d), and cell circularity (e). Data are shown as mean ± standard deviation from three independent experiments carried out on separate cultures. Data were statistically analysed by one-way ANOVA and Bonferroni’s post-hoc tests to compare all data sets. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. vehicle. The scale bar in a represents 10 μm