Fig. 7

Effect of CCR2 signaling on immune cell infiltration following brain irradiation. a eGFP+ CCR2-null chimeras were exposed to 0 or 35 Gy brain irradiation and sacrificed at 6 months post-radiation. The numbers of eGFP+ cells per square millimeter were calculated for three regions of interest (the fimbria/fornix, corpus callosum/extreme capsule, and hippocampus). Data were compared to eGFP+ CCR2-competent chimeras using a two-way ANOVA and Bonferroni post hoc tests. b Sections from eGFP+ CCR2-null chimeras versus eGFP+ CCR2-competent animals were stained immunofluorescently for CD3, MHC II, or CD11c. The number of positively stained cells also expressing eGFP was calculated for both the MHC II and CD11c analyses: total numbers of CD11c+ or MHC II+ cells are represented by the red bars, cells co-localizing with eGFP are represented by the green portion of the bars. All graph bars represent means ± SEM, n = 4–6 mice per condition: *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. For the percent of positively stained cells that also expressed eGFP, +p ≤ 0.05, +++p ≤ 0.001