Fig. 2
From: Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis
TLR2-dependent cocaine-mediated microglial activation. a Time-course of cocaine (10 μM) mediated induction of TNFα, IL-6, and MCP-1 mRNA expression in BV2 and mouse primary microglial cells by qRT-PCR. b BV2 and mouse primary microglial cells transfected with TLR2 siRNA but not si-Con resulted in abrogation of cocaine-mediated induction of TNFα, IL-6, and MCP-1 mRNAs by qPCR using 18S rRNA as internal control. c ELISA analysis revealed the concentrations of TNFα and MCP-1 proteins in the supernatants derived from BV2 cells knocked down for TLR2. All data are presented as mean ± SD of three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control group (Student’s t test)