Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis

Fig. 3

Cocaine-mediated up-regulation of TLR2 protein and microglial activation involves ROS. a ROS generation in BV2 cells exposed to cocaine assessed by DCFH-DA. b Effects of PBN (ROS scavenger) and APO (NADPH oxidase inhibitor) on cocaine induced ROS formation in BV2 cells. c Effects of PBN and APO on cocaine-mediated induction of TLR2 protein. TLR2 protein levels were measured at 12 h after cocaine treatment. For western blot analysis, the induction of TLR2 protein was normalized to β-actin. d Effects of PBN and APO on cocaine-mediated expression of TNFα, IL-6, and MCP-1 mRNAs. Relative levels of TNFα, IL-6, and MCP-1 mRNAs were analyzed by qPCR using 18S rRNA as an internal control. e Effects of PBN and APO on cocaine-mediated induction of TNFα and MCP-1 protein. ELISA analysis revealed the concentrations of TNFα and MCP-1 proteins in the supernatants of BV2 cells pretreated with PBN and APO followed by exposure to cocaine. All data are presented as mean ± SD of three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control group. # p < 0.05, ## p < 0.01, ### p < 0.001 vs cocaine group (Student’s t test)

Back to article page