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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis

Fig. 5

Role of ATF4 in cocaine-mediated up-regulation of TLR2 expression and microglial activation. a siRNA transfection was used to knock down ATF4. ATF4 siRNA but not si-Con inhibited cocaine-mediated induction of TLR2 protein. b Overexpression of ATF4 up-regulated TLR2 protein levels. c Role of ATF4 in cocaine-mediated induction of TNFα and MCP-1 mRNAs. d, e Cocaine-mediated nuclear translocation of ATF4 in BV2 cells. f Schematic illustration of ATF4 binding sequence on the promoter region of TLR2. ChIP assay demonstrating cocaine-mediated binding of ATF4 to the TLR2 promoter. All data are presented as mean ± SD of three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control group (Student’s t test)

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