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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis

Fig. 7

Cocaine-mediated up-regulation of both TLR2 and microglial activation in mouse microglia in vivo. a Chronic cocaine administration to mice resulted in significant induction of TLR2 protein in the striatum of mice compared with saline-injected controls. b Effect of cocaine on the expression of TLR2 and microglial activation in microglia isolated from the brains of cocaine/saline administered mice. Total RNA isolated from microglia was subjected to qPCR analyses using primer sets specific for TNFα, IL-6, MCP-1, and TLR2. c Floating tissue sections were double immunostained with antibodies specific for Iba1/TLR2 and assessed for co-localization of Iba1/TLR2 positive cells using the Nuance multi spectrum imaging system. Cocaine exposure resulted in increased numbers of TLR2 (red) positive microglia (Iba1, green) in the mouse brain. All data are presented as mean ± SD of three individual experiments. **p < 0.01, ***p < 0.01 vs saline group (Student’s t test)

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