Fig. 7

Macrophage depletion reduced microglia activation. a Photomicrographs represented Iba1 (green)-stained microglia in the cortex and striatum in CLP, PBS, and Vehicle groups at 14 days after MCAO. Right panels at each column (a2 to f2) presented a higher magnification of the images of left panels labeled by the boxes (a1 to f1). Scale bar = 100 μm in a1 to f1 and 20 μm in a2 to f2. b Quantification of the number of Iba1⁺ microglia located in the cortex (a) and striatum (c) and the percentage of Iba1⁺ /DAPI cells located in the cortex (b) and striatum (d) at 14 days after MCAO. Data are mean ± SD, n = 4 per group. *p < 0.05, **p < 0.01, CLP vs. Vehicle or PBS