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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model

Fig. 4

Effect of CD200Fc on CD200 and CD200R1 protein levels. Effect of CD200Fc on CD200 and CD200R1 protein levels of the L4–L5 spinal cord segments from sCCI-operated rats. sCCI of the sciatic nerve and ACSF treatment increased intensity of CD200 and CD200R immunofluorescences in DH of spinal cord sections when compared with those from sham-operated rats. Intrathecal CD200Fc administration significantly attenuated elevation of CD200 and CD200R immunofluorescences in the spinal cord sections of sCCI-operated rats (a–f). Scale bars = 100 μm. The CD200 and CD200R immunofluorescence changes were confirmed by western blot analysis of CD200 and CD200R proteins (g, h). Upper panels of g and h illustrate representative western blotting bands for CD200 and CD200R in the L4–L5 spinal cord segments from sham-operated (Sham), sCCI-operated and ACSF-treated (sCCI + ACSF5h) rats, as well as rats operated for sCCI and 5 (sCCI + CD200Fc5h) or 24 h (sCCI + CD200Fc24h) after administration of CD200Fc. Lower panels show mean density ± SE of individual CD200 and CD200R1 protein bands in triplicate analysis of six animals after normalization to tubulin. *p < 0.05 when compared to naïve rats; #p < 0.05 when compared to sCCI + ACSF rats; ++p < 0.05 when compared to 5 h after administration of CD200Fc. Mann-Whitney U test

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Journal of Neuroinflammation

ISSN: 1742-2094