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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model

Fig. 6

Double immunostaining to detect CD200R protein in astrocytes. Double immunostaining of section through DH of L4–L5 spinal cord segments from sCCI-operated and ACSF-treated rats to detect CD200R protein in GFAP positive astrocytes. Immunofluorescence staining for GFAP (a, d) and CD200R (b, e) was colocalized as shown in merged figure (c, f). Scale bars = 100 μm. d–f illustrate a higher power magnification of area limited by a box in c. Evidence of CD200R in activated astrocytes was confirmed by image analysis of three cells in the section using NIS Elements software. Mander’s overlap was 0.965265, 0.929336, and 0.960253 for cells indicated by arrows. Scale bars = 30 μm. g illustrates representative profile intensity of red (CD200R) and green (GFAP) channels in cell tagged 1

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