Fig. 1

Cauterization leads to an elevated IOP and RGCs death. a IOP monitoring of HT right eye, NT eye, and naïve eye over 40 days (n = 8 per group). b Peripheral area of whole flat-mounted retina marked with a white cross matching the region of interest. Whole flat-mounted retina immunolabeled with Brn3a antibody. Representative images of the peripheral area showing immunopositive staining of RGCs in a naïve eye, NT eye, and HT eye. Scale bar = 100 μm. c Quantification of peripheral RGC density per retina using automated nuclei counting in a naïve (n = 7), NT (n = 6), and HT eye (n = 6). Each density corresponds to an average of RGCs determined from eight peripheral images per retina. Data are expressed as means ± SEM. Two-way ANOVA for repeated measures followed by Bonferroni post hoc test was used for IOP: ****p < 0.0001 naïve and NT versus HT eyes. One-way ANOVA, followed by the Dunnett multiple comparisons test was used for RGC counting: *p < 0.05 naïve versus HT eyes