Episcleral vein cauterization experiments | RT-PCR | n = 8–10 rats | 2 | 20 |
Immunohistofluorescence | n = 6–7 rats | 4 | 28 | |
Stereotaxic CTB-Alexa 594 injection experiments | Histofluorescence | n = 4 rats | 1 | 4 |
Stereotaxic TNFα injection experiments | RT-PCR | n = 8–10 rats | 2 | 20 |
Immunohistofluorescence | n = 6 rats | 2 | 12 | |
Total number of rats | 84 |