Fig. 1

The lymphocyte populations in blood and cerebrospinal fluid of a TBE patient. The representative paired peripheral blood (PBMC—rows 1 and 3 from top) and csf (rows 2 and 4) samples collected from a TBEV-infected patient on admission to hospital were analyzed with Simultest IMK-Lymphocyte, as described in “Methods.” From the left to right in the upper two rows: the gated lymphocyte population; the negative control; identification of B (CD19+) and T (CD3+) lymphocytes; in the lower two rows: T CD3 + CD4+ lymphocytes; T CD3+CD8+ lymphocytes; and NK cells (CD3-CD16+CD56+). The csf lymphocyte population is enriched in CD3+CD4+ cells in comparison with the peripheral blood, at the expense of the other lymphocyte fractions, especially B lymphocytes